|
KMID : 1161420120150050490
|
|
Journal of Medicinal Food
2012 Volume.15 No. 5 p.490 ~ p.494
|
|
|
Antioxidant and Cytoprotective Effects of Oligomeric and Polymeric Procyanidin Fractions from Defatted Grape Seed in PC12 Cells
|
|
Kim Young-Hwa
Choi Young-Min
Ham Hyeon-mi
Jeong Heon-Sang
Lee Jun-Soo
|
|
|
Abstract
|
|
|
|
In this study, a crude polyphenol extract (CPE) from defatted grape seeds was evaluated for antioxidant and cytoprotective effects in PC12 cells. The CPE was then fractionated into a catechin and oligomeric procyanidin fraction (Fcat+olig) and a polymeric procyanidin fraction (Fpol), and the bioactivities of the fractions were evaluated individually. Fpol was the richest in total polyphenol, total flavonoid, and polymeric procyanidin but contained less flavan-3-ol than Fcat+olig. Consistent with these results, Fpol had the highest 2,2-diphenyl-β-picrylhydrazyl radical scavenging activity and reducing power. Both Fcat+olig and Fpol had higher 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic) acid radical scavenging activity than CPE. The resistance of PC12 cells against oxidative stress, after pretreatment with Fpol (200?μg/mL) for 9?h, was 100.7±2.9% of that in the control. Moreover, pretreating these cells with Fcat+olig and Fpol significantly decreased reactive oxygen species generation. These results provide information on a potential new source of antioxidants in the form of a functional food derived from an agricultural by-product.
|
|
|
KEYWORD
|
|
|
antioxidant, cytoprotective effects, grape seed meal, PC12 cells, procyanidin
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|